Douglas Vapor Batch Plate
The Douglas Vapor Batch Plate is designed for both microbatch and vapor
diffusion (sitting drop) crystallization. It has 96 wells in the center and
several reservoirs around the outside. The reservoirs can be used to
preserve microbatch crystals by preventing drying out of the wells, or they can
be used for simple vapor diffusion experiments where up to 96 wells are
equilibrated against a single precipitant (preliminary experiments suggest that
this method finds more hits in screening experiments than the conventional
method of using the same solution in the reservoir and the drop).
- Prevents leakage of oil from microbatch experiments
- Wells are individually labeled
- 96 wells (with a 4.5 mm spacing)
- Can be filled with 8-channel pipettes (transferring 6 drops at a time) by
filling odd numbered wells first, then even numbered wells
- Suitable for microbatch crystallization
- Suitable for vapor diffusion experiments where many drops are equilibrated
against one reservoir solution
- If crystals are not obtained in vapor diffusion experiments, the concentration of the reservoir solution
can easily be increased
- Because wells are smaller than Miniplates, HLA or Terazaki plates, they
are more suitable for drops below 0.5 microlitres
- Available as a hydrophilic version for screening or a hydrophobic version
for optimization. (The hydrophilic version gives better liquid
handling with small volumes, while the hydrophobic version reduces crystal
nucleation and helps to prevent the crystals from sticking to the plastic)
- If water or salt solution is placed in the reservoir wells of a microbatch
experiment this will prevent the wells from drying out. Microbatch
crystals will then last for up to several months.
- Suitable for the transport of crystals (plates can be turned upside down
without harming the drops)
Please click here for detailed instructions for
using the Douglas Vapor Batch Plate.
Holders are available from Douglas Instruments to provide a regular Linbro
or SBS ( i.e. regular 96-well) footprint.
The Douglas Vapor Batch Plate was designed and is manufactured by Douglas Instruments.
It can be obtained from Douglas Instruments and from Hampton
Douglas Instruments is running a competition
for the best use of the Douglas Vapor Batch Plate.
||Medical grade polystyrene
|Length of plate at the base
|Width of the plate at the base
|Height of the plate with the lid on
|Number of wells
|Shape of wells
|Width of well at the top
|Width of well at bottom
|Depth of well
|Volume of well
|Typical volume of drop used in wells
||0.4 microlitres ( i.e. 0.2 + 0.2) using robot, 2 microlitres
( i.e. 1 + 1) by hand.
|Minimum volume of drop used in wells
|Maximum volume of drop used in wells
||Approx. 20 microlitres.
|Number of reservoirs
||4 reservoirs, each divided into three or four
sections. (Reservoirs are in communication with all wells.)
|Volume of each section of reservoirs
|Total volume of reservoirs
For prices see the Products page.