Oryx Spec
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ORYX 6 Specification

July 2004

General

Crystallization Methods 1. Microbatch
2. Sitting Drop
Ambient temperature 4 to 30C
Computer requirement IBM Compatible (see separate specification)


Microbatch Method

Volume range of crystallization trial 0.2 to 10 microliters
Typical volume of crystallization trial for screening. 0.4 microliters
Typical volume of crystallization trial for optimization 2 microliters
Volume of protein dispensed per trial 0.1 to 5 microliters
Number of crystallization trials set up per run 1 to 192 (or more)
Number of solutions dispensed in each trial for optimization 1 to 5 (including water)
Plates accomodated All high quality plates can be used


Vapor Diffusion Method

Volume of droplet 0.2 to 10 microliters
Plates accommodated All high quality plates can be used
Number of wells dispensed 1 to 96


Microtips

Number of bores 5 or 2
Cross-section of microtip at tip 0.45 - 0.95 mm
Cross-section of each bore 0.15 mm I.D.
Dead volume Zero
Material Water repellent fluoropolymer.  Protein channel is treated to prevent the air-bubble from breaking up.


Universal Syringe Driver

Number of discrete steps for syringe volume More than 44,000
Displacement for each step 1.6 microns
Volume dispensed per step with a 100 microlitre syringe. 2.6 nl
Maximum speed 4.0 mm/second

Accuracy of Universal Syringe Driver
R.M.S. error per step +/- 8%
Nominal maximum error per step 16%
Nominal maximum cumulative error over complete linear displacement 2 steps


Automatic XYZV Plate Loader

Linear displacement of table:
travel, first horizontal axis (X) 321 mm
travel, second horizontal axis (Y) 151 mm
travel, first vertical axis (Z) 50 mm
travel, second vertical axis (Z) 52 mm
Nominal maximum cumulative error over complete linear displacement 0.1 mm
Length required on bench 670 mm
Depth required on bench 610 mm
Height in extended position 220 mm
Weight 11.8 kg
Time taken to dispense 96 well experiment into e.g Douglas Vapor Batch Plate 15 min
Maximum speed:
X 75 mm/s
Y 55 mm/s
Z &V 25 mm/s
The speed is software controllable and is fully variable


Each Oryx is controlled by one MCC Motion Control Center.  Click here for MCC specification.
 

                                                 2004 Douglas Instruments Ltd